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( A ) A significant reduction in the expression <t>of</t> <t>PLG</t> in PBMC of psoriasis patients was detected by flow cytometry. ( B ) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with a PLG mAb (red). ( C ) Lysates from lesional skin of psoriasis patients or normal skin of volunteers were subjected to western blotting for PLG expression. Human PLG was used as a positive control (right panel). Actin - loading control. ( D, E ) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with <t>uPA</t> or tPA mAbs (green) for its expression. Original magnification for immunofluorescent staining, ×400. e, epidermis; d, dermis. Dotted lines indicate the border between epidermis and dermis.
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( A ) A significant reduction in the expression of PLG in PBMC of psoriasis patients was detected by flow cytometry. ( B ) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with a PLG mAb (red). ( C ) Lysates from lesional skin of psoriasis patients or normal skin of volunteers were subjected to western blotting for PLG expression. Human PLG was used as a positive control (right panel). Actin - loading control. ( D, E ) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with uPA or tPA mAbs (green) for its expression. Original magnification for immunofluorescent staining, ×400. e, epidermis; d, dermis. Dotted lines indicate the border between epidermis and dermis.

Journal: PLoS ONE

Article Title: Plasmin Plays an Essential Role in Amplification of Psoriasiform Skin Inflammation in Mice

doi: 10.1371/journal.pone.0016483

Figure Lengend Snippet: ( A ) A significant reduction in the expression of PLG in PBMC of psoriasis patients was detected by flow cytometry. ( B ) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with a PLG mAb (red). ( C ) Lysates from lesional skin of psoriasis patients or normal skin of volunteers were subjected to western blotting for PLG expression. Human PLG was used as a positive control (right panel). Actin - loading control. ( D, E ) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with uPA or tPA mAbs (green) for its expression. Original magnification for immunofluorescent staining, ×400. e, epidermis; d, dermis. Dotted lines indicate the border between epidermis and dermis.

Article Snippet: The following antibodies were used for flow cytometry, all in conjunction with AlexaFluor 555 (Caltag Laboratories): anti-human PLG (Santa Cruz), anti-human uPA, anti-human uPA (R&D), and anti-human annexin A2 (ABGENT).

Techniques: Expressing, Flow Cytometry, Staining, Western Blot, Positive Control